Eclipse Ti2 Series

The ECLIPSE Ti2 inverted microscope delivers an unparalleled 25mm field of view (FOV) that revolutionizes the way you see. With this incredible FOV, the Ti2 maximizes the sensor area of large-format CMOS cameras without making compromises, and significantly improves data throughput. The Ti2's exceptionally stable, drift-free platform is designed to meet the demands of super-resolution imaging while its unique hardware-triggering capabilities enhance even the most challenging, high-speed imaging applications. Furthermore, the Ti2's unique, intelligent functions guide users through imaging workflows by gathering data from internal sensors, eliminating the possibility of user errors. In addition, the status of each sensor is automatically recorded during acquisition, providing quality control for imaging experiments and enhancing data reproducibility.

Groundbreaking FOV

As research trends evolve towards large-scale, systems-level approaches, there is an increasing demand for faster data acquisition and higher throughput capabilities. Development of large-format camera sensors and improvements in the data processing capabilities of PCs have facilitated such research trends. The Ti2, with its unprecedented 25mm field of view, provides the next level of scalability, enabling researchers to truly maximize the utility of large-format detectors and future-proof their core imaging platform as camera technologies continue to develop at a rapid pace.

Bright illumination over a wide area

High-power LEDs deliver bright illumination across the Ti2's large field of view, ensuring clear, consistent results from demanding applications such as high-magnification DIC. Incorporation of a fly-eye lens design provides uniform illumination from edge to edge for quantitative high-speed imaging and seamless tiling of images in stitching applications.

A compact epi-fluorescence illuminator designed for large FOV imaging is equipped with a quartz fly-eye lens and provides high transmittance across a broad spectrum, including UV. Large diameter fluorescence filters with hard coatings deliver large FOV images with a high signal-to-noise ratio.

Large diameter observation optics

The diameter of the observation light path has been enlarged in order to achieve a field number of 25 at the imaging port. The resulting large FOV is capable of capturing approximately double the area of conventional optics, enabling users to gain maximum performance from large-format sensors such as CMOS detectors.

Objectives for large FOV imaging

Objectives with superior image flatness ensure high quality images from edge to edge. Utilizing the maximum potential of the OFN25 objective significantly accelerates data collection.

Cameras for large-volume data acquisition

Nikon’s FX-format F-mount cameras Digital Sight 10 and DS-Qi2 are equipped with CMOS image sensors optimized for research use, originally developed for professional D-SLR cameras. This allows high-speed and high-sensitivity live-cell imaging, enabling the best use to be made of the Ti2’s large FOV.

Unsurpassed Nikon optics

Nikon's high-precision CFI60 infinity optics, designed for use with a variety of sophisticated observation methods, are highly regarded by researchers for their superb optical performance and solid reliability.

Apodized phase contrast

Nikon's unique apodized phase contrast objectives with selective amplitude filters dramatically increase contrast and reduce halo artifacts to provide detailed high-definition images.

External phase contrast

The motorized external phase-contrast system enables users to combine phase contrast with epi-fluorescence imaging without compromising fluorescent light transmission by bypassing the need to use phase-contrast objectives. For example, very high NA, liquid immersion objectives can be used for phase-contrast imaging. Using this external phase contrast system, users can easily combine phase contrast with other imaging modalities, including weak-fluorescence imaging such as TIRF and laser tweezer applications.

DIC (Differential Interference Contrast)

Nikon's highly-regarded DIC optics provide uniformly clear and detailed images with high resolution and contrast throughout the magnification range. DIC prisms are individually tailored for each objective lens to provide the highest-quality DIC images for every sample.

Leading platform for advanced imaging

• Bright and uniform illumination is provided across an unprecedented 25mm field of view that maximizes the sensor area of large-format CMOS cameras, and significantly improves data throughput
• Ti2-E is a motorized and intelligent model for advanced imaging applications, and Ti2-A and Ti2-U are manual models with imaging capability for laser applications. Ti2-A has unique, intelligent features
• Ti2-E is compatible with real-time focus maintenance Perfect Focus System (PFS), auto correction collar, and external phase contrast system
• For its stable and drift-free platform, Ti2-E is perfect for super-resolution and confocal imaging
• Ti2-E / Ti2-A s intelligent functions provide interactive guidance for microscope operation by integrating data from internal sensors, thus eliminating the possibility of user errors. The status of each sensor is automatically recorded during image acquisition

Technical Specifications

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