Lab on a Bead is a Swedish Biotech company that can provide the next generation high capacity magnetic separation platform for purification of biomolecules. The LOABeads magnetic beads combine the high binding capacity of agarose with fast magnetic separation. LOABeads are a flexible alternative for any bioseparation application, from microliter scale to liter scale.
Advantages of LOABeads :
- Superior Binding Capacity :
The high surface area of porous magnetic agarose beads results in high binding capacities. - Fast Magnetic Separation :
The LOABeads beads show excellent super-paramagnetic behavior and are strongly attracted to external magnets which enables separation within seconds. - High Specificity :
The hydrophilicity of the agarose offers minimal unspecific binding resulting in highly specific purifications.
LOABeadsTM Protein A :
These are intended for purification of antibodies from serum and ascites fluid samples, IgG serum depletion and immunoprecipitation. Combined with LOABeads MagSep separators, purification can be performed in standard 15 and 50ml centrifuge tubes, as well as 500ml bottles.
Product Data
| Product name | LOABeadsTM Protein A |
| Product number | 1001 |
| Particle size | 45-165 ?m |
| Ligand | Recombinant protein A |
| Type magnetization | Super-paramagnetic |
| Matrix | 4% agarose |
| Product form | 10% bead suspension in PBS witd 20% ethanol |
| Binding capacity* | 4 mg rabbit IgG/ml bead suspension |
| Max. binding capacity** | 6 mg human IgG/ml bead suspension |
| Binding buffer | PBS |
| Elution buffer | 60 mM citrate pH 3.0 |
| Storage conditions | +2 to +8?C in PBS witd 20% ethanol |
| Stability information*** | 24 montds |
| Regeneration | Can be reused multiple times |
* 90% binding of rabbit IgG (2 mg/ml) after 60 min reaction.
** Determined in an overloading test at 5 mg IgG/ml after 60 min reaction.
*** Data of product stability is continuously updated based on ongoing stability studies.
LOABeadsTM Affiamino :
LOABeadsTM AffiAmino are surface activated magnetic agarose beads employing Lab on a Bead?s patented coupling chemistry. Any amino containing ligand, e.g. proteins and antibodies can be covalently bound to the beads using a simple process.
Product Data
| Product name | LOABeadsTM AffiAmino |
| Product number | 1003 |
| Particle size | 45-165 ?m |
| Coupling to | Primary amino groups |
| Type magnetization | Super-paramagnetic |
| Matrix | 4% agarose |
| Product form | 10% bead suspension in PBS witd 20% ethanol |
| Coupling capacity* | 0.5-1 mg IgG/ml bead suspension 5-10 mg IgG/ml settled beads |
| Binding buffer | PBS witd 0.1% tween |
| Storage conditions | +2 to +8?C in PBS witd 20% ethanol |
| Stability information** | 12 months |
* 90% coupling of rabbit IgG (1 mg/ml) after 60 min incubation.
** Data of product stability is continuously updated based on ongoing stability studies.
LOABeadsTM MabBind A :
These are magnetic agarose beads coupled with protein A optimized for efficient purification of IgG from cell cultures. The high specificity and high capacity of LOABeads MabBind A ensures that a very pure antibody is obtained with a high yield, even when antibody capture is performed directly in the cell culture medium.
LOABeads MabBind A are ideal to use in many applications of magnetic bead affinity chromatography (MBAC). For example in lab-scale purification of antibodies to be used in R&D and diagnostics and for characterizations of candidate biopharmaceutical IgG antibodies.
Product Data
| Product name | LOABeadsTM MabBind A |
| Product number | 1004 |
| Particle size | 45-165 ?m |
| Ligand | Recombinant protein A |
| Type magnetization | Super-paramagnetic |
| Matrix | 4% agarose |
| Product form | 10% bead suspension in PBS witd 20% ethanol |
| Binding capacity* | 3 mg rabbit IgG/ml bead suspension |
| Binding conditions | Directly in cell culture media, pH 6?8 |
| Elution buffer | 60 mM citrate pH 3.0 |
| Storage conditions | +2 to +8?C in PBS witd 20% ethanol |
| Stability information** | 12 montds |
| Regeneration | Can be reused multiple times |
* Binding capacity was determined by incubating 500?l 10% LOABeads MabBind A (50 ?l beads) with rabbit IgG (?2 mg/ml in 1 ml PBS) for 60 minutes at room temperature. Binding capacity is obtained with a yield of 90% or more under the conditions specified above.
** Data of product stability is continuously updated based on ongoing stability studies.
LOABeadsTM MagSep :
These are durable separators for volumes between milliliter and liter. The LOABeads MagSep series of laboratory magnetic separators have been optimized for use with LOABeads magnetic beads and are ideal for most bioseparation applications.
Product Characteristics
| Product name | LOABeads? MagSep 15/50 | LOABeads? MagSep 500 |
| Product number | 3001 | 4001 |
| Diameter | 123 mm | 176 mm |
| Height | 119 mm | 148 mm |
| Weight | Approx. 400 g | Approx. 2000 g |
| Volume | 15 and 50 ml | 500 ml |
| Working volume | 3-50 ml | 250-500 ml |
| Working range beads* | 10 ?l-4 ml | 1-30 ml |
| Magnetic separation time | 10 seconds | 2-5 minutes |
* The recommended amount of settled LOABeads
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