The QuantaSep® 5000 system is mobile, compact, and capable of providing an operating flow range of 50ml-5L/min. The integrated system has two positive displacement pumps, 8 buffer selection valves, inline mixer, filters and 6 fractions collection valves. It has capabilities of buffer switching, gradient formation, feed pre-filtering, air ejection, conductivity and pH sensing pre and post column, forward and reverse flow in a powerful easy-to-use software package. The software enables buffer and fraction switching based on process conditions and alarms in the event of overpressure, air or leaks. All events are logged and archived.