Question 1

What is Centrifugation?

Accepted Answer

Centrifugation is the technique of separating components where the centrifugal force/ acceleration causes the denser molecules to move towards the periphery while the less dense particles move to the centre. Centrifugation is a technique used for the separation of particles from a solution according to their size, shape, density, viscosity of the medium and rotor speed. The process of centrifugation relies on the perpendicular force created when a sample is rotated about a fixed point.

Question 2

How Does A Centrifuge Work?

Accepted Answer

Centrifugation uses a centrifuge or a device that can rapidly spin, to speed up this process. Imagine we put the mixture into test tubes and those tubes into the centrifuge.

Question 3

What Are The Types of Centrifuges?

Accepted Answer

large-capacity low-speed preparative centrifuges preparative high-speed ultracentrifuges refrigerated preparative centrifuges/ultracentrifuges analytical ultracentrifuges large-scale clinical centrifuges small-scale laboratory microfuges.

Question 4

What is RCF?

Accepted Answer

Relative centrifugal force is the measure of the strength of rotors of different types and sizes. This is the force exerted on the contents of the rotor as a result of the rotation.

Question 5

What is RPM?

Accepted Answer

Revolutions per Minute (RPM) in regard to centrifugation is simply a measurement of how fast the centrifuge rotor does a full rotation in one minute.

Question 6

What is The Suitable RPM For My Application?

Accepted Answer

Centrifuges are used in various laboratories to separate fluids, gases, or liquids based on density. In research and clinical laboratories, centrifuges are often used for cell, organelle, virus, protein, and nucleic acid purification.

Question 7

What Are The Different Rotors?

Accepted Answer

Fixed angle Swinging bucket

Question 8

How Is A Fixed Angle Rotor Different Than A Swing Out Rotor?

Accepted Answer

The fixed-angle rotor is designed to hold tubes in a fixed position at a fixed angle relative to the vertical axis of rotation (up to about 45°).

Question 9

How Do I Choose The Correct RPM And Rotor?

Accepted Answer

If you follow a given protocol, make sure to use the same type of rotor and apply the given relative centrifugal force (rcf or g × force) as well as the same temperature and running time. In general, the following major parameters have to be determined to achieve a successful centrifugation run

Question 10

Do I Need A Refrigerated Centrifuge?

Accepted Answer

It is essential to select a centrifuge that is suited to the specific application. When purchasing a centrifuge, it is important to consider the following questions:

Question 11

How Is Lab-I-Fuge Unique?

Accepted Answer

To separate cellular and subcellular components, separating one cell type from another.Removing cells or other suspended particles from their surrounding milieu on either a batch or a continuous-flow basis.

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